すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. 01) + 0. The user manuals cover installation, troubleshooting, and validation of the SpectraMax M5 and its accessories. Microplate Readers include the M3, M4,. Table of Contents. Step 4: Incubate at room temperature for 1 hour, protected from light. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuidePlace the plate into a SpectraMax M5 e plate reader (Molecular Devices). Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. Fluorescent Polarization: Solid black plates, as above. 1. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. SPECTRAmax GEMINI-XS spectrofluorometer was able to detect a specific GFP signal at all cell numbers as determined by One Way Analysis of Variance (ANOVA) (Table 1). M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes. Microplate Readers Biotek Synergy H1 Hybrid Microplate Reader. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. Reduces well-to-well variability, improving assay quality (Z' factor) and reliability (CV %) of high-throughput. Add to Cart. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date: 5/20/2019 9:24:38 AM 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuide Place the plate into a SpectraMax M5 e plate reader (Molecular Devices). Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. BRET does not work on the Spectramax M5 due to a sensitivity issue and to the fact that the BRET assay has very weak signal in general. Molecular Devices Corporation have recently introduced the SpectraMax® M5e multi-detection microplate reader, an extension of the five-mode SpectraMax M5 system. When used with SoftMax® Pro Data Acquisition and Analysis Software, it can automatically normalize the well absorbance to a cuvette equivalentAnalytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Wypalls Paper Towel (Warehouse, Cat # 79300335 or equivalent) 7. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. Download PDF versions of the SpectraMax M5 User Guides from this page. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. For example, microplates containing different samples can be read using the312 gain calibration failed. M5. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Purchase Overlord with the required number of. . Sale. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. My experiments primarily involve monitoring the change in tryptophan fluorescence with time, using 96. View the Molecular Devices websiteResults by SpectraMax M5 from multiple independent donors are given. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Wide range of applications. Settings not required by a reader are indicated by ‘---’. D 1. The dual monochromator optics allow the widest range of applications to be utilized for bior. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. . DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. 3 or greater. . Our absorbance plate readers feature our PathCheck Sensor technology and. 0 out of 5 $ 17,999 $ 16,995. LMax, SpectraMax ®, VersaMax™, VMax , EMax , UVMax, FlexStation®, and ThermoMax benchtop Microplate Readers. Molecular Devices SpectraMax M5e User Manual (86 pages) Multi-Mode Microplate Readers. . Open the Softmax Pro software and select the FP option. . The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 315 can't find zero order. 6 Flexibility for your assay needs Robotics compatibility for increased throughput SpectraMax M Series readers can be easily integrated with our optional StakMax® Microplate Stacker forF1 SpectraMax Plus384andABSPlus: Certificate Value – 2≤ Average Peak Value≤ Certificate Value + 2 SpectraMax M2,M2e,M3,M4,M5,andM5e: Certificate Value – 3≤ Average Peak Value≤ Certificate Value + 3 Photometric Accuracy F2, F3, F4 AverageOD=Certificate Value± (Certificate Value(0. . 3. View the Molecular Devices websiteSpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. 72 MB. No filter change with dual monochromator optics. Fluorescence Microplate Reader Comparison Chart. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The chamber. Recertification is recommended every two years. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. Weight. . It should work on the Spectramax L reader and also on the Spectramax M3, M4, and M5 plate readers (new firmware only) with Softmax Pro ® software version 5. The test solution was by diluting FCV to PBS/ Glycerol solutions (5 μM, 1. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The M5 can read volumes down to 2uL with the. Note: In this user guide, all references to SpectraMax Multi-Mode. The guide also covers the software features, the specifications, and the accessories of the systems. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. no. Currently we have over 300 Windows 7/10 compatible instrument drivers to control equipment from 147 manufacturers, meaning that we are likely to have your instrument already in our library. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well Qualify the fluorescence performance of the SpectraMax . Molecular Devices. Figure 4: Transcreener ADP. . Note: for the THUNDER assay, performance of a SpectraMax M5e reader will be equivalent to that of a SpectraMax M5 reader. , ~488 nm), has the optical. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. Microplate Reader Shelf. D 1. Offer Ends In: 18 Days 19: 38: 21. SpectraMax®L Luminometer User Guide 0112-0174 B 11 The SpectraMax L Luminescence Microplate Reader can accommodate SBS-standard 96 and 384-well microplates with dimensions of 128. 303 unable to cal dark current. With optimized reagents, option to operate the readers. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. High tolerance for contaminants. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. 0 out of 5 $ 17,999 $ 16,995. The following document is intended to demonstrate setup of this instrument. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Cavicide (Warehouse, Cat # 79300360) 6. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Fluorescence spectra of CDs were measured using a microplate reader (SpectraMax M5, Molecular Devices). SpectraMax M5 device. Wavelength optimization. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. IP1 labeled with d2 acceptor competes with native (unlabeled) IP1 for binding to the donor-labeled IP1-specific monoclonal antibody. What can be done for Communication problems with new Windows 7 computer and SoftMax Pro 5. The purpose of the next experiment was to demonstrate that the Dual-Glo assay system could be used to measure the induction of pCREluc by forskolin. 303 unable to cal dark current. SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Add to Cart. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Optimizing Fluorescence Polarization Assays Fluorescence polarization for SpectraMax M5 and M5 readers may only be read from the top of a microplate. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax M5 Multi-Mode Microplate Reader. For example, microplates containing different samp les can be read using the same orProducts : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. D 1. 2. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. These all-inclusive assay kits are ready to use in assay validation or screening. Principle of the HTRF IP-One competitive binding assay. Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. The viscosity solution was prepared by mixing PBS and Glycerol in different proportion. clicked with the SpectraMax L Microplate Reader. . Exhibit with us {{>currencyLabel}} Back. Italic and boldface type are used for emphasis. 315 can't find zero order. 314 low lamp level warning. The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. IMAP Assay Kits IMAP Assay Kits provide a turnkey solution to evaluate . . SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax L Microplate Reader provides medium to high throughput. edu) to be added as a trained user in iLab. Devices SpectraMax i3, SpectraMax Paradigm, or SpectraMax M5 Multi-Mode Microplate Readers. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. All configurations offer a triple-mode cuvette port, accurate temperature control, microplate shaking and comprehensive data management using our SoftMax® Pro Microplate DataSpectraMax M3 Reader SpectraMax M4 Reader SpectraMax M5 Reader SpectraMax M5e Reader TRF,HTRF,FP HTRF, FP HTRF N/A Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well. Call 516-939-0888 to check current. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4 SpectraMax® M5 Multi-Mode Microplate Reader. View the Molecular Devices website. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. Molecular Devices SpectraMax PLUS 384 Spectrophotometer System-New ABs1 Performance Testing August 2021. The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Spectrophotometers for UV-Vis Absorbance Detection. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader Why does "Path?" appears in all wells when PathCheck is selected? Cause: Water Constants. The following document is intended to demonstrate setup of this. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Click SoftMax Pro Software User Guide. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Sensitive fluorescent quantitation of DNA with the Quant-iT. Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME. 1. For very concentrated samples, use a minimum2. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. SoftMax Pro can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change filters. Launch SoftMax® Pro Software and open the PicoGreen fluorescence protocol from the Protocols dropdown. . SpectraMax Quant dsDNA Assay Kits. The chamber. 1. -Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. The corrected absorbance is within 5% of the. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. SpectraMax Microplate Readers. The Spectramax can accommodate cuvettes or a variety of 6 to 96-well plate formats. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. In the M2, M5 and Plus 384, the light. Turn on the microplate reader. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax Lmicroplate luminometer. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. 5. Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax ® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. type Clear. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. General procedure forpreparedviscosity analysis The parent stock solution was prepared by dissolving probe FCV (5 mM) in DMSO. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Spectral Range. The following document is intended to demonstrate setup of this instrument. 1 or greater versions. Description. edu) to be added as a trained user in iLab. Fluorescence intensity was measured using a. . For more information please visit us at: Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. . Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. The large analog PMT works with monochromators and is used during fluorescence measurements or when a specific luminescence wavelength is selected as in the BRET. 1321N1, a human astrocytoma cell line, expresses. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. 1. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY. SpectraMax Injector Cartridge with SmartInject™ Technology. The SpectraMax® M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. . Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Wavelength (s) 200 to 1000 nm (Abs); 250 to 850 nm (Fluor); 250 to 850 nm (TRF) SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Description 1. The SpectraMax ABS Plus reader utilizes Molecular . 313 reference gain check fail. The REF button has no valid meaning for. . For more information, please visit us at Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. . Step 1: Open the SoftMax Pro IMAP FP protocol. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. If you do not have an iLab account you must first make one: Calcium Assay Kits are the platform of choice for measuring changes of intracellular calcium for drug discovery and basic research. Some luminescence applications, such as gene reporter assays, may require a luminometer with photon counting detection for greater sensitivity, such as the SpectraMax M5, Analyst GT or Analyst HT, or LMax II. 305 reference level saturation. The ability to measure inhibition of phosphorylation by Erlotinib was tested by incubating cells with increasing concentrations of Erlotinib, then stimulating cells with 0. Molecular Devices SpectraMax M5 Multi-mode Plate Reader 6. . Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideThe Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. Examples: “Press carefully to engage,” “ Do not. . The optical performance is comparable to a top-of-the-line dedicated The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. Statistical analyses The statistical analyses were performed using Graph Prism version 7. The. Spectramax M5 plate reader, 5 modes, 200-100 nm Abs range, Abs, FL, Lumi, TRF and FP, Monochromator in 1 nm increments, includes validation and warranty. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes 6. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). 多功能酶标仪 FlexStation3 快速动力学操. 6. SpectraMax ® M5 Multi-Mode Microplate Reader Transcreener ® ADP. The excitation and the emission wavelengths on. Products : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. HTRF is a proprietary time-resolved fluorescence technology that overcomesSpectraMax M5, Analyst HT or Analyst GT. The SpectraMax® Gemini™ XPS Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. 6. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. In the M2, M5 and Plus. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. . For example, microplates containing different samples can be read using theLocate the User Guide on your SoftMax Pro computer for version 6 and version 7. This analysis indicated that, after 48 hours incubation, there was a significant difference (P < 0. Figure 1. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed to be \~50 µg/mL for a 1-cm pathlength3,4. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers single. If you do not have an iLab account you must first make one: SpectraMax Paradigm Fluo rescence Polarization (FP) Detection Cartridge is a single slot detection cartridge and occupies one (1) slot. Molecular Devices SpectraMax 384 Plus Microplate Reader Specifications. SIRL-1 ligation reduced cytokines secretion from monocytes stimulated by NET. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. OptimizingAbsorbanceReadMode The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. 190-1000. Multimode Reader Comparison Chart. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Molecular Devices ®(MD) SpectraMax 340PC reader MD VersaMax™ Tunable Microplate readers MD SpectraMax® L Microplate Luminometers MD SpectraMax® M5 systems MD ®SpectraMax 96/384-well plate reader Gemini fluorescence plate reader Automation Hamilton Microlab® STAR™ liquid handling systemThe SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. . Sensitive fluorescent quantitation of DNA with the Quant-iT PicoGreen dsDNA Assay Kit The Spectramax M5 has similar detection capability to the Analyst GT and LMax II 384 (Figure 4), so we are confident that its performance is also similar when measuring the enzymes in cell lysates. As per Volza’s India Import data, Spectramax import shipments in India stood at 365, imported by 145 India Importers from 28 Suppliers. Molecular Devices SpectraMax M5/M5e Microplate Reader was tested for compatibility with Life Technologies LanthaScreen® Terbium-based TR-FRET assays. The day after, a final concentration of 1 μmol/L ARS1620, 20 nmol/L SMARTpool siRNA or the combination of the 2 was added in 5 replicates (wells). Issue: The audit trail in a SoftMax Pro GxP Software data file records a user as logging out of the system when the Auto Log Off prompt. Page: 1/6. Unit comes with software and computer. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific pro-teins, nucleic acids, and other molecules across a wide range of concentrations. The chamber contains. Cet appareil ISM est confomre à la norme NMB-001 du Canada. When the correct COM port is selected, the. We would like to show you a description here but the site won’t allow us. . HEALTH AND SAFETY CONSIDERAIONS StakMax Handling Stacker Base Unit. Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Also refer to this Multimode Reader Comparison Chart. KeySpan adapter for SoftMax Pro and SpectraMax readers. Pick the best speed, sensitivity, and price for your needs. . item 3 replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon replacement bulb for molecular devices spectramax xps xenon, spectramax xs xenon. Multimode Reader Comparison Chart. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Efects of luciferase concentration, luciferin concentration, ATP concentration, pH, temperature, and reaction time on the. 3. When the user applies a set temperature of 37°C, the top is heated to 37. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. A new microplate luminometry for the toxicity bioassay of chemicals on firefly luciferase, was developed using the multifunctional microplate reader (SpectraMax M5) to measure the luminous intensity of luciferase. Description 1. The plate reader. TR-FRET assay principle. 7. The microplates were read the next day from the bottom and the top in SpectraMax M5 and Gemini EM readers. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The SpectraMax M5 uses standard microplates to read endpoint, kinetic, spectrum, multi-wavelength and. Setup recommendations for SpectraMax® M5e. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. LMax and LMax II RLUs are proportional to (counts/sec) x (integration time), so for these readers if the signal is constant, doubling the integration time doubles the RLU. Two sequential measurements should be carried out: at 620 nm for the cryptate emission, and at 665 nm for the specific signal emitted by the acceptor. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. . type Clear. Please Inquire. The M5 can read volumes down to 2uL with the uMax Low Volume Plate. Note: SoftMax Pro Software version 6 and 7 also include a searchable help file within the software. AlphaScreen assays cannot be done on the SpectraMax M series, Gemini, and SpectraMax iDx (iD3/iD5) readers. The SpectraMax M2 and M2e are versatile and reliable. 1. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 2. In this. spr SpectraMax Plus 384. 306 plate air cal fail,low light. Graph standard curves and calculate sample protein concentrations easily with a preconfigured. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 2 SpectraMax MiniMax300ImagingCytometer(cytometer) 3. Measure assay absorbance values quickly with minimal background noise on the SpectraMax iD5 reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. PAINS analysisThe SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). com A. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Add to Cart. The plate reader can measure multiple readouts, including UV and visible absorbance. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Read More. Spectranet customer portalMice were fasted overnight and orally administered FITC-dextran (600 mg/kg). Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. 次いで、ユーザーのラボにとって最も役立つ機能を有するモデルを自由に. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. 1) SpectraMax Glo Steady Luc Reporter Assay (6. For example, microplates containing different samp les can be read using the same or Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax Plus 384 reader, SpectraMax 190 reader, VersaMax reader, FlexStation 3 reader I need to reinstall the USB driver for my plate reader. Recommended Optics Wavelength (nm) Wavelength selection Excitation 405/9 Monochromator Emission 1 450/15 Monochromator Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The following document is intended to demonstrate setup of this instrument and provide representativeThe SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. These lab workhorses are used in drug discovery, research, bioassay validation, and. 発光モードに対応した機種（SpectraMax M5e/M5/M4/M3）では、発光モード専用のフォトンカウント検出器も搭載しました。 蛍光に特化した検出器を流用するのではなく、専用の光学系を採用することで、発光、TR. 306 plate air cal. Molecular Devices SpectraMax 190 Microplate Absorbance Spectrophotometer. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) of samples in a microplate. 190 - 1100 nm. +1 800-635-5577. How do I do this? Note: Check the Applies to section at the bottom of the page to make this is the.